Next Generation Sequencing Facilitates Quantitative Analysis of ApoE-/-/SAHHWT, ApoE-/-/SAHH+/- mice and ApoE-/-/SAHH+/- +Betaine mice [RNA-seq]

Objective: the next generation sequencing (NGS) has revolutionized the system-based analysis of cellular pathways. The purpose of this study was to explore the mechanism of methylation changes caused by SAHH knockout and dietary betaine addition in atherosclerosis. Overall design: Methods: Mouse vascular tissue mRNA profiles of ApoE-/-/SAHHWT, ApoE-/-/SAHH+/- mice and ApoE-/-/SAHH+/- +Betaine mice were generated by deep sequencing, in triplicate. The library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated. Differential expression analysis of two conditions/groups was performed using the DESeq2 R package (1.20.0).