[RAW VALIDATED DATA] Properties of seawater and particulate matter from a WETLabs ALFA hyperspectral laser spectrofluorometer mounted on the continuous surface water sampling system during the Tara Oceans expedition 2009-2013@en

The Tara Oceans Expedition (2009-2013) sampled the world oceans on board a 36 m long schooner, collecting environmental data and organisms from viruses to planktonic metazoans for later analyses using modern sequencing and state-of-the-art imaging technologies. Tara Oceans Data are particularly suited to study the genetic, morphological and functional diversity of plankton. The present data set provides raw validated data of continuous measurements made with an Aquatic Laser Fluorescence Analyzer (ALFA) (Chekalyuk et al., 2014), connected in-line to the TARA flow through system during 2013. The ALFA instrument provides dual-wavelength excitation (405 and 514 nm) of laser-stimulated emission (LSE) for spectral and temporal analysis. It offers in vivo fluorescence assessments of phytoplankton pigments, biomass, photosynthetic yield (Fv/Fm), phycobiliprotein (PBP)-containing phytoplankton groups, and chromophoric dissolved organic matter (CDOM) (Chekalyuk and Hafez, 2008; 2013A). Spectral deconvolution (SDC) is used to assess the overlapped spectral bands of aquatic fluorescence constituents and water Raman scattering (R). The Fv/Fm measurements are spectrally corrected for non-chlorophyll fluorescence background produced by CDOM and other constituents (Chekalyuk and Hafez, 2008). The sensor was cleaned weakly following the manufacturer recommended protocol.塔拉海洋科考航次（Tara Oceans Expedition）于2009至2013年间搭乘一艘36米长的纵帆船对全球海洋开展采样工作，收集了从病毒到浮游多细胞动物（planktonic metazoans）的各类生物与环境数据，以供后续借助现代测序技术与前沿成像技术开展分析。塔拉海洋数据集（Tara Oceans Data）尤其适用于研究浮游生物的遗传、形态与功能多样性。本数据集提供了水生激光荧光分析仪（Aquatic Laser Fluorescence Analyzer, ALFA）（Chekalyuk等，2014）所测得的经过验证的连续原始数据，该仪器于2013年通过在线方式连接至塔拉号（TARA）流通系统。ALFA仪器采用双波长激发（405 nm与514 nm）的激光诱导发射（laser-stimulated emission, LSE）技术，用于光谱与时域分析。其可实现浮游植物色素、生物量、光合产率（Fv/Fm）、含藻胆蛋白（phycobiliprotein, PBP）的浮游植物类群以及有色溶解有机物（chromophoric dissolved organic matter, CDOM）的活体荧光评估（Chekalyuk与Hafez，2008；2013A）。光谱反褶积（spectral deconvolution, SDC）可用于解析水体荧光组分与水拉曼散射（R）的重叠光谱波段。针对有色溶解有机物及其他组分产生的非叶绿素荧光背景，研究人员对光合产率（Fv/Fm）的测量值进行了光谱校正（Chekalyuk与Hafez，2008）。该传感器按照制造商推荐的规程进行了轻度清洁。