Whole transcriptome RNA sequencing of E16.5 Six2TGC and Six2TGC;miR-17~92fl/fl nephron progenitors. Mus musculus

The goal of this study is to identify dysregulated mRNAs and lncRNAs in nephron progenitors as a consequence of a loss of miR-17~92 post-transcriptional regulation Overall design: Nephron progenitors were FACS sorted from E16.5 Six2TGC (Control) and Six2TGC;miR-17~92fl/fl (Mutant) kidneys based on the GFP driven by the Six2 locus. A total of 3 replicates per genotype were used for the whole transcriptome RNA sequencing. Each replicate consists of nephron progenitors pooled from 4 embryos, resulting in 12 samples per genotype assayed in this manner.