A single-cell analysis reveals tumor heterogeneity and immune environment of acral melanoma [RNA-Seq]

Acral melanoma is considered as a special subtype of melanoma. We performed 11 single cell RNA sequencing (scRNA seq), 57 Bulk RNA sequencing, 8 whole exon sequencing(WES seq), and 6 TCR sequencing to analyze the tumor heterogeneity and immune environment characteristics of acral melanoma. Overall design: We used the single cell data of 5 cases of acral melanoma and 3 cases of cutaneous melanoma as the discovery set for comparative analysis, and used 57 cases of Bulk-RNA data and 3 cases of single cell RNA data to verify the results. 8 WES data were used to analyze the mutation characteristics of the discovery set samples, and 6 TCR data were used to analyze the T cell clone diversity of the two subtype melanoma samples.