Bulk RNA-seq of CRISPRi ADGRG6-kd iAT2 following cigarette smoke exposure

We performed bulk transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of ADGRG6. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Cells were plated at an air-liquid interface, then subsequently exposed to air or 5% cigarette smoke using a VitroCell smoke robot. Cells were harvested for bulk RNA sequencing 8 hours post cigarette smoke exposure Overall design: Bulk transcriptomic analysis of iPSC-derived type 2 alveolar epithelial cells (iAT2) comparing wild-type cells with CRISPRi-mediated knockdown of ADGRG6 following exposure to air or 5% cigarette smoke exposure