Microarray analysis of inflammatory gene expression during culture of RAW 264.7 macrophages in tumor cell conditioned buffer.

CT26 tumor cell conditioned buffer was applied to RAW 264.7 cells for 24 hrs prior to mRNA isolation and analysis using the Codelink Mammalian Inflammation Bioarray. Significance of transcript upregulation or downregulation was determined using the VAMPIRE statistical algorithm. Data represents the fold change in transcript expression from 3 separate experiments. Of the 270 genes detected in RAW 264.7 cells (Supplemental Table S1), the most highly upregulated or downregulated genes associated with migration, proliferation and angiogenesis are presented.