Sequences of Peptides and Oligonucleotides.
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*The HTHi peptide reproduces the Gly-149 to Lys-186 sequence of HIV-1 IN Phe185Lys [20]. The loop region is underlined. The mutated residues are in bold characters. An N-terminal labeled carboxyfluorescein derivative of HTHi was also used (not shown). The peptides α4, K156 and INH5 were dealt with in [30], [43], [50].**The LTR34 oligonucleotides are designed to adopt a hairpin conformation, as already reported [43]. The hairpins contain a 17 bp stem, reproducing the HIV-1 U5LTR end, and a loop formed by three thymine nucleotides (TTT in bold characters). The fluorescein reporter group, f, is grafted either to the 5′ extremity (LTR34f5′, CRE) or to the central T residue (F) of the loop (LTR34fm).
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2015-12-02



