Figure 2B middle AJ 160628, 160705, 160718

This dataset comprises TIF files representing acquisitions in one single molecule experiment. There are two types of file. Each TIF file contains a stack of images representing a time series at a single field of view. An accompanying text file indicates the laser in use at each frame. In this case, the exposure time for each frame was 20 msec. Illumination was by laser 2 (640 nm, Cy5) from time frames 3 to 102 and laser 1 (488 nm, mEGFP) from frames 103 to 242. Deviation (Dev) represents the deviation of the exiting laser as a result of autofocussing from the point at which all four quarters of a quartered photodiode were irradiated with equal intensity. The experiment was done by Andrew Jobbins.