The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 17 h

To delineate cellular processes involved in NiV FG combination and mutants with different fusiogenecity, we have employed whole genome microarray expression profiling as a discovery platform to identify the upregulated and downregulated genes. Total RNA was collected from transfected cells and untransfected controls. Cy3 labeled cRNA is used for hybridization platform Agilent Human 4 x 44K gene expression array. FG and mutants transfections were performed at 17h. These transfected cells were compared to untransfected HEK293T cells. Five replicates were performed for each condition.