Structural analysis of dimeric calreticulin using stable isotope labelling, cross-linking, mass spectrometry and modelling

Description of raw-files:

FUS04612: Orbitrap Fusion analysis of heat-treated and BS3 cross-linked dimeric calreiticulin where the dimer is a combination of human recombinant 15N-labelled calreticulin expressed in P. pastoris mixed with native human placental calreticulin.

FUS04614: Duplicate of FUS04612

QHF1883: Q Exactive HF analysis of heat-treated and BS3 cross-linked dimeric calreiticulin where the dimer is a combination of human recombinant 15N-labelled calreticulin expressed in P. pastoris mixed with native human placental calreticulin.

QHF1884: Duplicate of QHF1883

Obvb08125: Orbitrap Velos Pro analysis of heat-treated and BS3 cross-linked dimeric calreiticulin where the dimer is a combination of human recombinant 15N-labelled calreticulin expressed in P. pastoris mixed with native human placental calreticulin.

Obvb08126, Obvb08127, Obvb08128: Three replicates of Obvb08125.

原始文件描述：
FUS04612：利用 Orbitrap Fusion 对经热处理和 BS3 交联的二聚体钙网蛋白进行 Orbitrap Fusion 分析，其中该二聚体由表达于毕赤酵母中的人源重组 15N 标记的钙网蛋白与原代人胎盘钙网蛋白的混合物组成。
FUS04614：FUS04612 的副本
QHF1883：利用 Q Exactive HF 对经热处理和 BS3 交联的二聚体钙网蛋白进行 Q Exactive HF 分析，其中该二聚体由表达于毕赤酵母中的人源重组 15N 标记的钙网蛋白与原代人胎盘钙网蛋白的混合物组成。
QHF1884：QHF1883 的副本
Obvb08125：利用 Orbitrap Velos Pro 对经热处理和 BS3 交联的二聚体钙网蛋白进行 Orbitrap Velos Pro 分析，其中该二聚体由表达于毕赤酵母中的人源重组 15N 标记的钙网蛋白与原代人胎盘钙网蛋白的混合物组成。
Obvb08126, Obvb08127, Obvb08128：Obvb08125 的三个重复样本。