OMIP-047: High-dimensional phenotypic characterization of B cells

Study the dynamics of B cell subsets and their phenotype in chronic viral diseases
Conclusion:
This panel allows the identification of all major B cell subsets. The phenotypic profile encompassing chemokine receptors, immunoglobulin isotypes, IL-21R and the proliferation marker Ki67 show sufficient resolution in order to determine the phenotypic characteristics of B cell subsets. Quality control of the BD Fortessa flow cytometer was regularly performed using the BD CS&T module. Antibodies were titrated to determine the optimal staining index and if necessary antibody concentration was adjusted to reduce spillover-dependent spreading. FMO controls were used to determine expression of dimly expressed markers.