OMIP-047: High-dimensional phenotypic characterization of B cells

Study the dynamics of B cell subsets and their phenotype in chronic viral diseases Conclusion: This panel allows the identification of all major B cell subsets. The phenotypic profile encompassing chemokine receptors, immunoglobulin isotypes, IL-21R and the proliferation marker Ki67 show sufficient resolution in order to determine the phenotypic characteristics of B cell subsets. Quality control of the BD Fortessa flow cytometer was regularly performed using the BD CS&T module. Antibodies were titrated to determine the optimal staining index and if necessary antibody concentration was adjusted to reduce spillover-dependent spreading. FMO controls were used to determine expression of dimly expressed markers.本研究旨在探究慢性病毒性疾病中B细胞亚群及其表型的动态变化。 结论： 本流式检测panel可实现对所有主要B细胞亚群的鉴定。涵盖趋化因子受体、免疫球蛋白同种型、IL-21R以及增殖标志物Ki67的表型谱具备足够分辨率，可用于明确B细胞亚群的表型特征。研究中定期通过BD CS&T模块对BD Fortessa流式细胞仪开展质量控制。通过对抗体进行滴定以确定最优染色指数，必要时调整抗体浓度以降低荧光渗漏导致的信号扩散。采用荧光减一（FMO，Fluorescence Minus One）对照来确定低表达标志物的表达情况。

搜集汇总

背景与挑战

背景概述

该数据集名为'OMIP-047: High-dimensional phenotypic characterization of B cells'，专注于研究慢性病毒疾病中B细胞亚群的高维表型特征，通过流式细胞术分析趋化因子受体、免疫球蛋白同种型等标记，以识别B细胞亚群并评估其表型动态。数据集包含健康捐赠者和HIV+个体的样本，采用严格的质量控制措施，如FMO对照和抗体滴定，MIFlowCyt得分为84.00%，表明数据注释质量较高。