Myelin like electrogenic filamentation and Liquid Microbial Fuel Cells Dataset overview.

Biofilm at water-oil interface of hypoxic water columns of microcosms, prepared from a lacustrine sample, that used diesel as a carbon source was found to show electrogenic properties. These microcosms named, liquid microbial fuel cells (L-MFCs) were electrically characterized using a custom electronic analyzer; accurate determination of voltage (V), power density (W/m 2), and current density (A/m2) for both charge and discharge phases was carried out. The instrument made it possible to carry out cell characterizations using resistive loads between 0 Ω (Ohm) and 10 kΩ. During the hypoxic and electrogenic phase, the synthesis of a system of “bacterial piping induction”, produced filaments of hundreds of micrometers in which the microbial cells are hosted. Ultrastructural microscopy collected by scanning (SEM), transmission (TEM), immunofluorescence, Thunder Imager 3D, confocal laser scanning (CLSM) microscopy revealed a “myelin like“ structure during filamentation processes; this “myelin like” structure exhibited cross-reactivity towards different epitopes of the myelin basic protein (MBP) and Claudin 11 (O4) of human oligodendrocytes. The disclosure of these filamentation processes could be helpful to describe further unconventional microbial structures in aquatic ecosystems and of the animal world.以湖泊样本制备的微宇宙（microcosms）缺氧水柱的水油界面生物膜，以柴油作为碳源时，被证实具有产电特性。该类微宇宙被命名为液态微生物燃料电池（liquid microbial fuel cells, L-MFCs），采用定制电子分析仪进行电学表征；实验精准测定了充放电阶段的电压（V）、功率密度（W/m²）与电流密度（A/m²）。该仪器可实现0Ω（欧姆）至10kΩ范围内不同阻性负载下的电池表征。在缺氧及产电阶段，"细菌管道诱导"系统的合成过程会生成数百微米级的丝状结构，微生物细胞定植于其中。通过扫描电子显微镜（scanning electron microscopy, SEM）、透射电子显微镜（transmission electron microscopy, TEM）、免疫荧光技术、Thunder Imager 3D成像系统及共聚焦激光扫描显微镜（confocal laser scanning microscopy, CLSM）采集的超微结构显微数据显示：在丝状形成过程中存在"髓鞘样"结构；该结构可与人少突胶质细胞的髓鞘碱性蛋白（myelin basic protein, MBP）以及Claudin 11（O4）的不同表位发生交叉反应。揭示此类丝状形成过程，有助于进一步阐释水生生态系统及动物界中的非常规微生物结构。