Microbial Observatory at North Temperate Lakes LTER High-resolution temporal and spatial dynamics of microbial community structure in freshwater bog lakes 2005 - 2009 original format

The North Temperate Lakes - Microbial Observatory seeks to study freshwater microbes over long time scales (10+ years). Observing microbial communities over multiple years using DNA sequencing allows in-depth assessment of diversity, variability, gene content, and seasonal/annual drivers of community composition. Combining information obtained from DNA sequencing with additional experiments, such as investigating the biochemical properties of specific compounds, gene expression, or nutrient concentrations, provides insight into the functions of microbial taxa. Our 16S rRNA gene amplicon datasets were collected from bog lakes in Vilas County, WI, and from Lake Mendota in Madison, WI. Ribosomal RNA gene amplicon sequencing of freshwater environmental DNA was performed on samples from Crystal Bog, North Sparkling Bog, West Sparkling Bog, Trout Bog, South Sparkling Bog, Hell’s Kitchen, and Mary Lake. These microbial time series are valuable both for microbial ecologists seeking to understand the properties of microbial communities and for ecologists seeking to better understand how microbes contribute to ecosystem functioning in freshwater.北温带湖泊-微生物观测站（North Temperate Lakes - Microbial Observatory）旨在开展为期10年以上的长期淡水微生物研究。通过DNA测序对微生物群落进行多年观测，可对群落的多样性、变异性、基因组成，以及驱动群落组成的季节与年度变化因素开展深度解析与评估。将DNA测序获得的信息与补充实验（如探究特定化合物的生化特性、基因表达或营养盐浓度）相结合，可深入阐明微生物类群的生态功能。本研究的16S rRNA基因扩增子（16S rRNA gene amplicon）数据集采集自威斯康星州维拉斯县的沼泽湖泊，以及威斯康星州麦迪逊市的门多塔湖。研究团队对采集自水晶沼泽（Crystal Bog）、北闪光沼泽（North Sparkling Bog）、西闪光沼泽（West Sparkling Bog）、鳟鱼沼泽（Trout Bog）、南闪光沼泽（South Sparkling Bog）、地狱厨房（Hell’s Kitchen）以及玛丽湖（Mary Lake）的淡水环境DNA样本，完成了核糖体RNA基因扩增子测序。上述微生物时间序列数据集，对于致力于解析微生物群落特性的微生物生态学家，以及旨在深入理解微生物如何助力淡水生态系统功能运行的生态学家而言，均具备极高的科研价值。