Gene expression profiling of IL7R+PIR+ and IL7R-PIR- subsets in LIN-c-Kit+ E12.5 mouse fetal liver cells

In order to find out target genes of Runx/Cbfb2 complexes in PIR+IL7R+ subset. In particular, genes that would explain why the size of thymus was small by lack of Cbfb2. Upon finding of decreased IL7R+PIR+ subset, which was reported as thymus-homing progenitor, in Cbfb2 deficient embryos, we focused on these population. Examination of wild type and knock out in 2 cell types.本研究旨在鉴定PIR+IL7R+细胞亚群中Runx/Cbfb2复合物的靶基因，尤其聚焦于可解释Cbfb2缺失引发胸腺体积缩小的相关基因。我们在Cbfb2缺陷胚胎中发现IL7R+PIR+细胞亚群的丰度显著降低，而该亚群此前已被报道为胸腺归巢祖细胞，因此将研究对象锁定于此细胞群，并针对两种细胞类型分别开展野生型与基因敲除型的检测分析。