Identifying Putative Targets of Capn15 in Neurodevelopment

Calpain 15 (CAPN15), part of the non-classical SOL family, plays a key role in neurodevelopment. This study used RNA-seq in P2 mice (7 KO and 7 WT) to investigate transcriptomic changes after loss of CAPN15. Differential expression analysis identified Pax2 targets as significantly regulated in CAPN15 KO mice. This work provides insight into CAPN15's molecular mechanisms and its potential role in human developmental disorders. Overall design: Fourteen P2 mouse brains (7 KO, 7 WT) were sequenced using Illumina NovaSeq 6000. RNA-seq analysis involved ribodepletion, Trimmomatic trimming, STAR alignment, and edgeR for differential expression analysis.