Single cell RNA sequencing of adult regenerating mouse digit tips

Innate regeneration following digit tip amputation is one of the few examples of epimorphic regeneration in mammals. Digit tip regeneration is mediated by the blastema, the same structure invoked during limb regeneration in some lower vertebrates. By genetic lineage analyses in mice, the digit tip blastema has been defined as a population of heterogeneous, lineage restructed progenitor cells. These previous studies, however, do not comprehensively evaluate blastema heterogeneity or address lineage restruction of closely related cell types. Here we report one additional single cell RNA sequencing sample (28 days post-amputation) to add to our already published 38,000 cells from mouse digit tip blastemas and unamputated control digit tips used to generate an atlas of the cell types participating in digit tip regeneration. Overall design: Adult wildtype FVB/NJ mice used to generate 28 days post-amputation digit tips for single cell RNA sequencing by 10X genomics. GSM4276219, GSM4276220, GSM4276221, GSM4276222, and GSM4276223 from GSE143888 were re-used for the current study