Audit-ready dataset of seven isolates (2015) from Cyrtomium fortunei leaf tissue documenting endophytes (plant-associated bacterial and fungal communities) by culture and Sanger barcoding

Background: Plant-associated microbiomes are increasingly recognized as essential components of ecosystem function and plant health, yet baseline data for non-model hosts remain scarce. Ferns represent an under-sampled lineage, and apogamous (asexual) ferns provide a relatively stable host-genetic background that can facilitate future, comparable microbiome studies. Methods: On 16 May 2015, leaf tissue of the apogamous fern Cyrtomium fortunei was collected from genetically confirmed isolates. Leaf segments were sequentially surface-sterilized with 70% ethanol and sodium hypochlorite and rinsed with sterile distilled water. Sterile segments were plated on potato dextrose agar (Nissui Pharmaceutical, Tokyo, Japan) and incubated at 25°C until microbial colonies emerged. Genomic DNA extraction and PCR amplification followed standardized protocols in the Japanese Pharmacopoeia (15th edition) . Fungal and bacterial isolates were barcoded by Sanger sequencing of ITS1 and partial 16S rRNA gene fragments, respectively. Taxonomic assignment was performed by BLASTn against the NCBI nucleotide database on 04 Aug 2015, and genus-level calls are reported conservatively. Results: Eleven cultivable isolates were obtained; seven yielded interpretable barcode sequences that were deposited in DDBJ/GenBank (LC750340.1–LC750346.1). These seven isolates comprised five fungi (ITS1) and two bacteria (16S rRNA). Genus-level assignments included Annulohypoxylon (three isolates), Colletotrichum (one isolate), Aspergillus (one isolate), and Paraburkholderia (two isolates). To maximize auditability without additional experiments, we provide a complete trace-to-result archive including AB1 chromatograms, chromatogram PDFs, derived FASTA sequences, and BLAST top-hit tables, organized by sample.