NEON Biorepository Mammal Collection (Pathogen Extracts [Ear Tissue])

<p>This collection contains genetic extracts from ear punch (tissue) samples that were collected from select small mammals during small mammal sampling and sent for testing for tick-borne pathogens (NEON sample class: rpt2_pathogentesting_in.earExtract). Small mammal sampling is based on the lunar calendar, with timing of sampling constrained to occur within 10 days before or after the new moon. Typically, core sites are sampled 6 times per year, and relocatable sites 4 times per year. Small mammals are sampled using box traps (models LFA, XLK, H.B. Sherman Traps, Inc., Tallahassee, FL, USA). Box traps are arrayed in three to eight (depending on the size of the site) 10 x 10 grids with 10m spacing between traps at all sites. Small mammal trapping bouts are comprised of one or three nights of trapping, depending on whether a grid is designated for pathogen sample collection (3 nights) or not (1 night). A subset of captured small mammals have tissues sampled (ear punch) for archive, DNA analysis or pathogen testing. Ear punches are collected once per life of an individual. Ear samples are placed on dry ice in the field and stored at -80 degrees Celsius. Ear tissue samples are tested via PCR individually for a variety of tick-borne pathogens. Pathogen extracts from the University of Massachusetts Laboratory of Medical Zoology are archived at the NEON Biorepository in 2 mL cryovials and stored at -80 degrees Celsius. See related links below for protocols and NEON related data products.
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本集合汇集了从小型哺乳动物耳部组织样本中提取的遗传物质，这些样本在小型哺乳动物采样期间收集，并送往检测蜱传病原体（NEON样本类别：rpt2_pathogentesting_in.earExtract）。小型哺乳动物的采样工作基于月相，采样时间限定在新月前后10天内进行。通常，核心采样点每年采样6次，可移动采样点每年采样4次。小型哺乳动物通过箱式陷阱（型号LFA、XLK、H.B. Sherman Traps，位于美国佛罗里达州塔拉哈西）进行采样。箱式陷阱在各个采样点以10米间距排列成3至8个（根据采样点规模而定）10x10的网格。小型哺乳动物的采样周期由一晚或三晚组成，具体取决于是否指定网格用于病原体样本收集（三晚）或非指定（一晚）。部分捕获的小型哺乳动物进行组织样本（耳部穿刺）的采集，用于存档、DNA分析或病原体检测。耳部穿刺样本在采集后置于干冰上，并在现场以-80摄氏度保存。耳部组织样本通过PCR技术单独检测多种蜱传病原体。来自马萨诸塞大学医学动物学实验室的病原体提取物存档于NEON生物样本库的2毫升冷冻管中，并储存在-80摄氏度。有关协议和NEON相关数据产品，请参阅以下相关链接。