RNA-Seq on Primary Mouse Hepatocytes BA condition
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https://zenodo.org/record/10492590
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After extractions, cells were cultured in 5% CO2 at 37°C using Williams E media supplemented with 2.24 g/l NaHCO3, 100 U/100 µM penicillin/streptomycin, 100 µM dexamethasone and 2 mM L-glutamine.
RNA was isolated using the RNeasy® Mini Kit (Qiagen, 74104) on-column DNA digestion was performed.
Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs GmbH, Germany)
BA treatment was performed during 48h.
Genome assembly: mm10
创建时间:
2024-01-12



