Proteins Under Pressure: Osmotic Pressure of Bespoke Proteins
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https://doi.esrf.fr/10.15151/ESRF-ES-2208654109
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资源简介：
Within cells, crowding by numerous biomacromolecules impacts protein stability. The primary crowding agents are other proteins, occupying up to 30% of the volume, but most studies use model crowders like polyols, sugars, and Ficoll. While these experiments offer insight into the molecular forces governing protein crowding, their biological relevance is limited. To bridge this gap, we designed bespoke proteins of varying lengths but with similar surfaces. We propose SAXS measurements to determine the osmotic pressure of solutions of these proteins at varying concentrations and temperatures. Osmotic pressure determination requires low-q measurements and accurate background subtraction, both achievable at ESRF beamlines. These experiments will allow to refine our theoretical model, enhancing our understanding of protein crowding by other proteins. Our work will elucidate interactions crucial for cellular function and the preservation of proteins in the food and pharmaceutical industries.
提供机构：
Hebrew University of Jerusalem, Institute of Chemistry, Givat Ram Safra, 91904 , Jerusalem, ISRAEL; University of North Carolina, Department of Chemistry CB#3290, NC, 27599-3290, Chapel hill, USA; Hebrew University of Jerusalem, Institute of Chemistry, Givat Ram Safra, 91904 Jerusalem, Israel
创建时间：
2028-01-01
