CIL:50453, Mus musculus, Mouse brain tissue. In Cell Image Library
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https://library.ucsd.edu/dc/object/bb2021184z
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Brief description of sample preparation
After cryofixation by high-pressure freezing and freeze-substitution, cryofixedmouse brain slices were rehydrated gradually. Rehydrated samples were then imaged with confocal microscopy to capture the DRAQ5 and tdTomato signals. Next, the samples were stained using a high-contrast en bloc staining protocol. Then the samples were dehydrated for resin infiltration and embedding, followed by imaging with X-ray microscopy and then SBEM.
Confocal Microscopy
After freeze-substitution and rehydration, the mouse brain slice was placed in ice-cold 0.15 M sodium cacodylate for imaging. Confocal volumes of DRAQ5 and tdTomato signals were collected on an Olympus FluoView 1000 confocal microscope with a 20X air and 60X water objectives using 561 nm and 633 nm excitation.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
