Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa

Phloem proteins (P-proteins) may play crucial role in phloem transporting cell function. We used single-nucleus RNA sequencing (snRNA seq) to analyze P-protein expression in Populus trichocarpa root. Cell nuclei were isolated from pioneer root fragments of Populus trichocarpa, stained with DAPI and sorted using a BD FACSAria™ Fusion flow cytometer