Gene expression profiles of murine fibroblasts and iPS

We reprogrammed adult murine fibroblasts obtained from p14f/f (wt version of conditional loxP-flanked p14 knockout (KO); C57/Bl6) by transduction of a lentiviral vector (pRRL.PPT.SF.hOKSMco.idTom.PRE) overexpressing the four Yamanaka factors (c-Myc, Klf-4, Oct-4, Sox-2). After successful reprogramming and establishment of stable iPS clones we performed gene expression profiling of three different murine iPS clones (#2, #2EX, #3) and their parental fibroblasts. murine adult fibroblasts from ear were isolated, taken into culture and transduced with pRRL.PPT.SF.hOKSMco.idTom.PRE After successful reprogramming total RNA from three independent iPS clone and their parental fibroblast was extracted and subjected to Microarray Analysis on Affymetrix Mouse GeneChip1.0 ST Arrays.