Pseudogene Recoding Revealed from Proteomic Analysis of Salmonella Serovars

Recoding refers to the reprogramming of mRNA translation
by nonstandard
read-out rules. In this study, we used stable isotope labeling with
amino acids in cell culture (SILAC) technology to investigate the
proteome of host-adapted Salmonella serovars, which
are characteristic of accumulation of pseudogenes. Interestingly,
a few annotated pseudogenes were indeed able to express peptides downstream
of the inactivation site,
suggesting the occurrence of recoding. Two mechanisms of recoding,
namely, programmed frameshifting and codon redefinition, were both
found. We believe that the phenomena of recoding are not rare in bacteria.
More studies are required for a better understanding of bacterial
translation and the implication of pseudogene recoding in Salmonella serovars.

重编程指通过非标准读取规则对mRNA翻译进行重新编码。在本研究中，我们运用细胞培养中稳定同位素标记氨基酸（SILAC）技术，探究了宿主适应性沙门氏菌血清型的蛋白质组，这些血清型以伪基因积累为特征。有趣的是，一些已标注的伪基因确实能够在失活位点下游表达肽段，这表明了重编程现象的发生。我们发现了两种重编程机制，即程序化移码和密码子重新定义。我们坚信，重编程现象在细菌中并不罕见。为进一步深入了解细菌翻译机制以及伪基因重编程在沙门氏菌血清型中的意义，需要开展更多研究。