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METHOD FOR BREEDING SELF-COMPATIBLE POTATOES

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as S s11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

创建时间:
2023-02-12
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