Transcriptomic analysis of gnotobiotic Aedes aegypti mosquitoes

We compare the transcriptome of gnotobiotic Ae. aegypti generated by contaminating axenic (bacteria-free) larvae with bacterial isolates found in natural mosquito breeding sites. We focused on four bacterial isolates (Lysobacter, Flavobacterium, Paenibacillus and Enterobacteriaceae) and found that different gnotobiotic treatments resulted in massive transcriptomic changes throughout the mosquito development. 48 samples, 3 mosquito stages (larval, pupal and adult stages), 5 conditions, 3 replicates of each type of sample. The non-axenic samples were the reference sample at each stage.