Motor neuron diseases, such as amyotrophic lateral sclerosis (ALS) and progressive bulbar palsy, involve loss of muscle control resulting from death of motor neurons. Although the exact pathogenesis o
The goal of the experiment is to identify genes involved in T-cell receptor (TCR) signaling and T-cell activation in human T cells. The Jurkat E6 cell line, engineered to express dCas9-KRAB and transd
We compared changes in sgRNA abundance between T24 cells challenged and unchallenged by CD8+ T cells, and then performed negative screening (p < 0.01, log2Fold change < −1.0, and good sgRNAs ≥ 5). (X
Investigating Bcor target genes in BcorΔE9-10KrasG12D leukaemia with a custom CRISPR dropout screen BcorΔE9-10KrasG12D cell line was transduced with a custom CRISPR library and a dropout screen was pe
Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine. For understanding pluripotency and lineage differentiation mechanisms,
