Chimeric contribution of human extended pluripotent stem cells to monkey embryos

Pluripotent stem cells (PSCs) represent the earliest stages of organismal development, and have the potential to differentiate into cell types from all three primary germ layers. To date, however, chimeric competency of human EPS cells has not been determined in other animal hosts. Leveraging on a recently developed in vitro culture system that enabled the development of monkey embryos up to 20 days in vitro, in this study, we injected human PSCs (hPSCs) cells into cynomolgus monkeys (Macaca fascicularis) morula embryos and examined their contribution to different lineages at different timepoints during in vitro culture. We first injected human PSCs (hPSCs) cells into cynomolgus monkeys. Then in vitro culture of chimeric embryos to study chimeric contribution of human extended pluripotent stem cells to monkey embryos