Structural Analyses of CEP Adducts.
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A) SDS-PAGE analysis. Aliquots of HSA-CTL1 (untreated), HSA-CTL2 (treated but unadducted) and HSA-CEP were subjected to reducing SDS-PAGE on 4–10% gels. Compared to HSA-CTL1, both HSA-CTL2 and HSA-CEP showed an increase in high-MW bands. B) Size exclusion chromatography. SEC under non-denaturing conditions indicated an increase in faster-eluting peaks in HSA-CTL2 and HSA-CEP compared to HSA-CTL1. C) Circular dichroism. CD analysis revealed a loss of secondary structure in HSA-CTL2 and HSA-CEP compared to HSA-CTL1.
创建时间：
2016-02-23
