The difference of gene expression for live or dead BbrY associated mice.. Mus musculus

To confirm epithelial gene expression of the large intestine after inoculation of dead (heat-treatment) or live Bifidobacterium probiotic strain (BbrY) we have employed whole genome microarray expression profiling. The epithelial cells were released from dead or live BbrY associated mice 3 or 28 days after association and GF mice with HANKS including EDTA and Hepes and treated by TRI-zol reagent. It was confirmed that the live BbrY associated mice were affected epithelial gene expression much more than dead cell feeding by K-means clustering and the functional categories. Overall design: BbrY-association induced gene expression in cecal epithelial cells was measured at 3 or 28 days after BbrY-association. Four mice was used for each experiment and GF mice were used as reference.