Single cell sequencing of stromal vascular fraction from facial infiltrating lipomatosis and normal facial adipose tissue

Facial infiltrating lipomatosis is characterized by excessive growth of adipose tissue, The etiology is associated with somatic PIK3CA variant, but the specific mechanisms are not yet fully understood. In this study, we collected facial adipose tissue from both FIL patients and non-FIL individuals, isolated the stromal vascular fraction (SVF) and performed single-cell transcriptome sequencing on these samples. We mapped out the cellular landscape within the SVF and specifically focused on a deeper analysis of fibro-adipogenic precursor cells (FAPs). Overall design: Adipose tissues from four FIL patients and three patients underwent facial cutaneous lesions were included in the single- cell sequencing experiments.