Supplementary Table S1. Differentially-expressed genes in glomeruli isolated from NPHS2-Cre +/+ versus wild-type (WT) mice

To investigate differential gene expression that might account for the differing glomerular phenotype of NPHS2-Cre +/+ mice when compared with wild-type control mice, RNA sequencing analysis was performed on glomeruli extracted from both NPHS2-Cre +/+ and wild-type control mice. 230 genes were identified that were considered to be significant, differentially expressed between the groups, meeting an FDR value of < 5% (q value < 0.05) and fold change ≥ ± 2 cut-off (Supplemental Table S1). Of these significant, differentially regulated genes, 139 were up-regulated and 91 were down-regulated in glomeruli of NPHS2-Cre +/+ mice as compared with that seen in wild-type control mice. To better understand their biological significance, gene ontology analysis was performed using both PANTHER and DAVID classification systems. The biological processes predominantly reflected by the significant, differentially regulated genes included cellular process (44.4%), metabolic process (28.0%), biological regulation (19.3%), response to stimulus (16.4%), localization (12.1%), developmental process (10.6%), and cellular component organization or biogenesis (10.6%), with some overlap across categories. Less associated biological processes (<10%) included multicellular organismal process, biological adhesion, immune system process, locomotion, reproduction, and growth. Some genes were not categorized by the PANTHER and DAVID software and were classified as “uncharacterized.”为探究可解释NPHS2-Cre+/+小鼠与野生型对照小鼠肾小球表型差异的差异基因表达情况，研究人员对提取自两组小鼠的肾小球开展了RNA测序（RNA sequencing）分析。最终共筛选得到230个组间差异表达显著的基因，这些基因满足错误发现率（False Discovery Rate，FDR）<5%（q值<0.05）且倍数变化（fold change）≥±2的筛选阈值（详见补充表S1）。在上述显著差异表达基因中，与野生型对照小鼠相比，NPHS2-Cre+/+小鼠肾小球内有139个基因表达上调，91个基因表达下调。 为进一步明确这些差异基因的生物学意义，研究人员分别采用PANTHER与DAVID分类系统开展了基因本体分析（Gene Ontology analysis）。显著差异表达基因所富集的主要生物学过程包括：细胞过程（44.4%）、代谢过程（28.0%）、生物学调控（19.3%）、应激应答（16.4%）、定位（12.1%）、发育过程（10.6%）以及细胞组分组织或生物发生（10.6%），各分类间存在一定重叠。占比低于10%的关联较弱的生物学过程则包括多细胞生物过程、生物黏附、免疫系统过程、运动、繁殖及生长。部分基因未被PANTHER与DAVID软件注释分类，被归类为“未表征基因”。