Targeted DamID to understand transcription factor genomic occupancy in human foetal lung progenitor cells

In order to study the genomic occupancy of transcription factors, including SOX9, ETV4 and ETV5, in human foetal lung tip progenitor cells, we have introduced Targeted DamID system via lentivirus in a human foetal lung tissue derived organoid culture (Nikolic et al., 2017). We were able to identify the corresponding TF motif enrichment and discovered a potential co-regulation function of SOX9 and ETV factors in human feotal lung progenitor cells. This study has pioneered the use of targeted DamID approach in tissue derived organoid system. Overall design: We used 4 different independent organoid lines treated as replicates, all of which are around 8 pcw. We introduced the SOX9 targeted DamID into organoids via lentiviral transduction and compared with the results from organoids with Dam only controls. In a following-up experimental setting, we introduced ETV4 and ETV5 targeted DamID into organoids via lentiviral transduction and compared with the results from organoids with Dam only controls.