Pioneer factors govern super-enhancer dynamics in stem cell plasticity and lineage choice

Mouse hair follicles undergo synchronized cycles. Cyclical regeneration and hair growth is fueled by hair follicle stem cells (HFSCs) and transit-amplifying cells (TACs). We used ChIP-seq to unfold genome-wide chromatin landscapes of H3K27ac and Med1 to identify super-enhancers and dissect their biological relevance in cell identity and plasticity of HFSCs in vivo and in vitro. Telogen quiescent hair follicle stem cells (HFSCs) and transit-amplifying cells (TACs) were FACS-purified for ChIP-sequcencing. Furthermore, cultured HFSCs were used for ChIP-sequencing.