Fudan: ASH1 3'UTR level 0 sequencing file
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This part is for the construction of our composite part (BBa_25AT6YR4) as a 3'UTR to guide mRNA to locate in the daughter cells, ensuring continuous and uninterrupted expression of the fluorescent protein and guaranteeing the continuity and accuracy of timekeeping (Bertrand et al., 1998) . It is a Level 0 of the Golden Gate assembly cloning method (Lee et al. 2025). There are more details on our Design page.In this dataset, we provided the ab1 sequencing result and genbank file with features of this level 0.Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS synthetic biology, 4(9), 975–986. https://doi.org/10.1021/sb500366v Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H., & Long, R. M. (1998). Localization of ASH1 mRNA particles in living yeast. Molecular cell, 2(4), 437–445. https://doi.org/10.1016/s1097-2765(00)80143-4



