Doryteuthis pealeii embyronic transcriptome individual stages 16-27 pooled and annotation

Photoreception is a ubiquitous sensory ability found across the Metazoa, and photoreceptive organs are intricate and diverse in their structure. Although the morphology of the compound eye in Drosophila and the single-chambered eye in vertebrates have elaborated independently, the amount of conservation within the 'eye' gene regulatory network remains controversial, with few taxa studied. To better understand the evolution of photoreceptive organs, we established the cephalopod Doryteuthis pealeii as a lophotrochozoan model for eye development. Utilizing histological, transcriptomic and molecular assays, we characterize eye formation in Doryteuthis pealeii Through lineage tracing and gene expression analyses, we demonstrate that cells expressing Pax and Six genes incorporate into the lens, cornea and iris, and the eye placode is the sole source of retinal tissue. Functional assays demonstrate that Notch signaling is required for photoreceptor cell differentiation and retinal organizatio...光感受（Photoreception）是后生动物（Metazoa）界广泛分布的感知能力，光感受器官的结构既复杂精巧又种类繁多。尽管果蝇（Drosophila）的复眼与脊椎动物的单腔眼在形态上各自独立演化形成，但“眼”相关基因调控网络（gene regulatory network）的保守程度仍存在争议，目前已开展研究的类群仍十分有限。为更深入地解析光感受器官的演化机制，我们选取头足类动物皮氏枪乌贼（Doryteuthis pealeii）作为冠轮动物（lophotrochozoan）类群中眼发育研究的模式生物。我们通过组织学、转录组学（transcriptomic）与分子实验（molecular assays）技术，对皮氏枪乌贼的眼发育过程进行了系统表征。通过谱系示踪（lineage tracing）与基因表达分析实验，我们证实表达Pax基因与Six基因的细胞会整合至晶状体、角膜与虹膜中，且眼板（eye placode）是视网膜组织的唯一来源。功能实验结果表明，Notch信号通路（Notch signaling）对于光感受器细胞的分化与视网膜组织的构建是必需的……