Tissue-specific long read RNA sequencing of Lumbricus rubellus.

Cwmystwyth is an abandoned lead mine site. The soil of this metalliferous mine site is inhabited by lead hyper-resistant Lumbricus rubellus earthworms. The nature of their resistance to heavy metal lead has not been elucidated in fine detail, especially within a tissue-specific context. Moreover, earthworms have yet to be studied using long read transcriptomic technologies. Hence, various tissues of a Cwmystwyth earthworm were dissected and long read RNA sequenced using the PacBio Sequel Ile system. Overall design: Various tissues of a clade “B” Lumbricus rubellus earthworm were RNA extracted and long read RNA determined using PacBio HiFi sequencing.