Code for the article "Sequence-dependent biomolecular phase separation driven by short-ranged interaction: From material properties to coarsening dynamics"

Liquid-liquid phase separation (LLPS) of biomacromolecules drives the formation of biomolecular<br>condensates, which possess material properties crucial for various biological functions. While recent<br>studies have primarily focused on LLPS driven by long-ranged, non-specifc interactions, the role of<br>short-range, one-to-one specifc interactions in sequence-dependent behavior still remains elusive. In<br>this study, we combined theoretical analysis and coarse-grained molecular dynamics simulations to<br>systematically investigate the sequence-dependent material properties of biomolecular condensates.<br>By introducing the sequence descriptor ϕ∗, we identifed strong correlations between ϕ∗ and key<br>material or structural properties, such as the single-chain radius of gyration, critical temperature,<br>density, surface tension, viscosity, and diffusion coefcient. Notably, near critical points, surface<br>tension and viscosity exhibit distinct scaling relationships with temperature, with viscosity showing<br>much greater sensitivity. Additionally, we found that sequences with high ϕ∗ may impede the<br>efcient growth of droplets. Our fndings provide a framework for understanding sequence-dependent<br>material properties and offer valuable insights into designing biomolecular condensates with tailored<br>stability and dynamic functionality.<br>