Ig repertoire of IgG+ murine memory B cells

BCR repertoire sequencing of isotype-switched memory B cells Aim of the study was to explore the differences of Ig repertoires of isotype-switched memory B cells between spleen and bone marrow compartments. Overall design: Cells from BM (tibiae, femurae and pelvic bones) and spleen of three times NP-CGG immunized-mice were isolated. Sorted cells of Mouse 1 and 2 were split into two equal aliquots (samples A, B) for BM and spleen samples as cellular (biological) replicates. Sorted cells of Mouse 3 were split into 4 samples (A, B, C, D). Biological replicates were processed independently from this point on. Isolated RNA was split and library preparation was performed in technical duplicates.