Infectivity of PySPZ inoculated IV.

A sample of 15 mosquitoes was randomly taken from the same container in which the mosquitoes used to bite mice in experiment #5 were taken. Salivary glands were dissected from the 15 mosquitoes and sporozoites isolated. The indicated numbers (first column) were injected IV into mice. 7 days and 14 days later, the presence of parasitemia was determined by microscopic evaluation of thin blood smears. The ID50 was calculated, and determined to be 1.09 PySPZ.