16S eubacterial PCR ONT sequencing evaluation on clinical samples

This study establishes and validates a standardized 16S nanopore sequencing (Oxford Nanopore Technologies, ONT) workflow for routine clinical diagnostics, benchmarking its performance against an Illumina NGS pipeline (MiSeq, 2x250bp). Detection limits were first assessed using dilution series of pure and mixed bacterial cultures (E. coli, S. aureus), followed by validation in 101 clinical samples, from routine diagnostics.