Data for: Evaluation of transcriptional biomarkers using a high-resolution regression approach: Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)

Data for the paper 'Evaluation of transcriptional biomarkers using a high-resolution regression approach: Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)'. ABSTRACT: We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13–1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38–160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress. Full reference: Ekelund Ugge, G.M.O., Jonsson, A., Walstad, A., Berglund, O. (2022). Evaluation of transcriptional biomarkers using a high-resolution regression approach: Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina). Environ Toxicol Pharmacol, 90, 103795. https://doi.org/10.1016/j.etap.2021.103795本数据集关联论文《使用高分辨率回归方法评估转录生物标志物：铜暴露淡水蚌（Anodonta anatina）中选定转录本的浓度依赖性》。 摘要：本研究针对选定的基因转录本（cat、gst、hsp70、hsp90、mt与sod）的浓度依赖性展开测试，以评估其作为化学胁迫生物标志物的潜力。与常规采用因子设计与少量暴露浓度的研究思路不同，本研究针对高分辨率浓度梯度开展回归分析。具体而言，将淡水蚌（Anodonta anatina）进行急性（96小时）铜暴露实验，共设置13个名义浓度（实测浓度范围为0.13~1600 μg/L），并通过逆转录实时定量聚合酶链反应（RT-qPCR）对转录本进行定量检测。在消化腺中，cat、hsp90与mt的表达量随水体铜浓度升高而下调（p < 0.05），但响应幅度趋于饱和，下调倍数不足2倍。在鳃组织中，gst、hsp70、hsp90与mt的表达量随水体铜浓度升高而上调（p < 0.05）。尽管在暴露浓度范围内，hsp70、hsp90与mt的上调倍数超过2倍，但所需铜浓度较高（38~160 μg/L）。尽管鳃组织的响应整体上较消化腺更为显著，但整体响应幅度较小且灵敏度适中，这可能限制了其作为通用化学胁迫生物标志物的应用潜力。 完整参考文献： Ekelund Ugge, G.M.O., Jonsson, A., Walstad, A., Berglund, O. (2022). 使用高分辨率回归方法评估转录生物标志物：铜暴露淡水蚌（Anodonta anatina）中选定转录本的浓度依赖性. 环境毒理学与药理学, 90, 103795. https://doi.org/10.1016/j.etap.2021.103795