File S1 - The Ca2+-Activated K+ Channel KCa3.1 as a Potential New Target for the Prevention of Allograft Vasculopathy

Supporting Table 1. Percentage of luminal occlusion and intima/media ratios evaluated at three different levels of each harvested graft in the different treatment groups. Supporting Table 2. Averaged adventitia, media and intima areas measured at three levels in each harvested graft are given in µm2. Total mononuclear cell numbers were determined in the same sections. Supporting Figure 1. KCa3.1 expression in rat vasculopathy. (A-D) Serial sections stained with H&E or antibodies against α-SMA, the macrophage marker ED1 ( = CD68) and KCa3.1. Sections A, B, C and D are sequential and each section is 5 µm from the previous section. (E) KCa3.1 expression on the vascular endothelium of an isograft. (F) Close-up of the boxed area in E. Supporting Figure 2. Specificity of Kv1.3 antibody (Sigma P9170) in mouse and rat spleen. (A, B) Sequential rat spleen sections stained with the polyclonal anti-Kv1.3 antibody (P9170; 1:750) or secondary antibody only. (C, D, E) Kv1.3 staining in mouse spleen. C and D are wild-type mouse spleen sections stained with anti-Kv1.3 (1∶750) or secondary antibody only. E shows a Kv1.3 knock-out mouse spleen section stained for Kv1.3 (1∶750). In all sections secondary antibody binding is visualized by DAB. Sections are counterstained with hematoxylin. (PDF)