Amine metabolites in pigs fed a diet with spray dried plasma protein as functional protein source

We evaluated the effects of diets formulated with either soybean meal (SBM) as a reference protein source or SDPP in pigs. Blood amine profiles were analysed to evaluate the effects of the diets at a systemic level. Blood samples were collected via the ear-vein for  plasma preparation at at dissection days (d28-29) after the morning meal ingestion. 

For plasma, blood samples were collected in sterile Vacuette tubes containing lithium-heparin and immediately centrifuged at 3,000x g for 10 min at 4°C and plasma was extracted. Plasma were stored at -80°C for further analysis on levels ofsystemic amine metabolite profiles. 

The protocol outlined in the following publication was used for detecting plasma amine levels:

Noga MJ, Dane A, Shi S, Attali A, van Aken H, Suidgeest E, et al. Metabolomics of cerebrospinal fluid reveals changes in the central nervous system metabolism in a rat model of multiple sclerosis. Metabolomics. 2012;8(2):253-63.

van der Kloet FM, Bobeldijk I, Verheij ER, Jellema RH. Analytical Error Reduction Using Single Point Calibration for Accurate and Precise Metabolomic Phenotyping. Journal of Proteome Research. 2009;8(11):5132-41.