Loss of dihydroceramide desaturase drives neurodegeneration by disrupting endoplasmic reticulum and lipid droplet homeostasis in glial cells. Loss of dihydroceramide desaturase drives neurodegeneration by disrupting endoplasmic reticulum and lipid droplet homeostasis in glial cells

We performed high-throughput RNA sequencing to determine the transcriptional changes of infertile crescent (ifc) mutants as compared to wild-type controls in the dissected CNS in Drosophila melanogaster. Overall design: We dissected and extracted RNA from 20-30 CNS of third instar larve for wild type or ifc mutant per sample, with five total samples per experimental group.