MiCroKitS

offline During cell cycle, numerous proteins temporally and spatially localized in distinct sub-cellular regions including centrosome (spindle pole in budding yeast), kinetochore/centromere, cleavage furrow/midbody (related or homolog structures in plants and budding yeast called as phragmoplast and bud neck, respectively), telomere and spindle spatially and temporally. These sub-cellular regions play important roles in various biological processes. In this work, we have collected all proteins identified to be localized on kinetochore, centrosome, midbody, telomere and spindle from two fungi (S. cerevisiae and S. pombe) and five animals, including C. elegans, D. melanogaster, X. laevis, M. musculus and H. sapiens based on the rationale of "Seeing is believing" (Bloom K et al., 2005). Through ortholog searches, the proteins potentially localized at these sub-cellular regions were detected in 144 eukaryotes. Then the integrated and searchable database MiCroKiTS - Midbody, Centrosome, Kinetochore, Telomere and Spindle has been established.

在细胞周期中，众多蛋白质在细胞的不同亚细胞区域进行时空定位，包括中心体（在酵母菌中称为纺锤极）、着丝粒/着丝粒、分裂沟/中体（在植物和酵母菌中分别称为细胞板和芽颈的同源结构）、端粒和纺锤体。这些亚细胞区域在多种生物过程中扮演着关键角色。在本研究中，我们根据‘眼见为实’（Bloom K 等人，2005年）的原则，收集了来自两种真菌（酿酒酵母菌和刚果酵母菌）以及五种动物（秀丽隐杆线虫、黑腹果蝇、非洲爪蟾、小鼠和人类）中定位在着丝粒、中心体、中体、端粒和纺锤体上的所有蛋白质。通过直系同源搜索，检测到这些亚细胞区域可能定位的蛋白质在144种真核生物中。随后，我们建立了综合可搜索数据库MiCroKiTS - 中体、中心体、着丝粒、端粒和纺锤体。

搜集汇总

背景与挑战

背景概述

MiCroKitS是一个专注于细胞周期中蛋白质定位的数据库，包含来自多种生物体的87,983个独特蛋白质条目。该数据集由中国的非营利机构维护，数据开放获取，但目前处于离线状态。