Proteomic data of dilution series of bacteria and diatoms using MS (DDA).

<p>To mimic a complex marine sample, a dilution series of R. pomeroyi and T. pseudonana was created at different cellular ratios. These mixtures were filtered and proteins were extracted from the filter for tryptic digestion and LC-MS/MS analysis.</p> <p>Biological fractions were lysed, digested and analyzed using proteomic mass spectrometry.</p> <pre> Data are available for download at the EBI PRIDE Archive. Homepage: <a href=\"https://www.ebi.ac.uk/pride/archive\" target=\"_blank\">https://www.ebi.ac.uk/pride/archive</a> Project URL: <a href=\"https://www.ebi.ac.uk/pride/archive/projects/PXD004799\" target=\"_blank\">https://www.ebi.ac.uk/pride/archive/projects/PXD004799</a> Data URL: <a href=\"https://www.ebi.ac.uk/pride/archive/projects/PXD004799/files\" target=\"_blank\">https://www.ebi.ac.uk/pride/archive/projects/PXD004799/files</a> </pre>为模拟复杂海洋样本，我们以不同细胞比例构建了R. pomeroyi与T. pseudonana的梯度稀释混合体系。将上述混合体系进行过滤，并从滤膜中提取蛋白质，用于胰蛋白酶消化及液相色谱-串联质谱（LC-MS/MS）分析。 生物组分经裂解、消化后，采用蛋白质组质谱技术开展分析。 数据集可于欧洲生物信息研究所（EBI）PRIDE 数据库下载获取： 主页：<a href="https://www.ebi.ac.uk/pride/archive" target="_blank">https://www.ebi.ac.uk/pride/archive</a> 项目链接：<a href="https://www.ebi.ac.uk/pride/archive/projects/PXD004799" target="_blank">https://www.ebi.ac.uk/pride/archive/projects/PXD004799</a> 数据下载链接：<a href="https://www.ebi.ac.uk/pride/archive/projects/PXD004799/files" target="_blank">https://www.ebi.ac.uk/pride/archive/projects/PXD004799/files</a>