Gene expression changes in SHI-1 cells following exposure to disulfiram/copper, niclosamde or combined drugs

Disulfiram and niclosamide were identified as drugs that induced depletion of an MLL-AF9-luciferase fusion protein in THP-1 AML cells, in a bioluminescence sreen. MLL-fusion protein depletion was confirmed in AML and ALL cell lines expressing different MLL-fusion proteins. Combination of disulfiram with niclosamide was found to enhance depletion of MLL-fusion proteins. To investigate whether this enhanced depletion resulted in increased suppression of downstream target genes. MLL-AF6 expressing SHI-1 cells were exposed to disulfiram/copper, nciclosamide or combined drugs. SHI-1 cells were treated for 16 hours with 0.3uM disulfiram / 1uM copper, 5uM niclosamide or combined drugs and RNAseq performed on isolated RNA. Overall design: Total cellular RNA was purified from SHI-1 cells treated with DMSO, 0.3uM disulfiram / 1uM copper, 5uM niclosamide or combined drugs for 16 hours, from three independent experiments