(a) Primer list used to clone greater amberjack leptin; (b) Comparable sequence from closely related species for primer design; (c) Primers for greater amberjack real-time PCR.

NIAID Data Ecosystem50

Oligonucleotide primers used in the amplification of the protein coding sequences in this study.

基因克隆引物设计

分子克隆

The underlined nucleotides indicate overlapping base pairs with the generic primers for the ORFs cloned using the Gateway® system. The bold underlined nucleotides show the restriction site for the app

NIAID Data Ecosystem30

Primers for 18S rRNA cloning and for real-time RT-PCR.

实时荧光定量PCR

基因克隆引物设计

“Accession Number” refers to the accession for the sequence in NCBI (18S rRNA) or the UF ProbeName for the corresponding microarray probe. Transcripts: Ctr1c, copper transporter 1c; TepII, thiolester

NIAID Data Ecosystem60

A novel global cDNA amplification technology directly from an RNA template via reverse transcription

单细胞转录组学

cDNA扩增技术

We developed a method, Reverse Transcription with Random Displacement Amplification (RT-RamDA), to reverse transcribe and amplify cDNA directly from RNA templates using strand displacement and RNA-dep

NIAID Data Ecosystem40

Primers for Amplification of cDNA Encoding CvIV4.

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